NLS Peptide Improves the Efficiency of pDNA Delivery into Eukaryotic Cells by Cationic Liposomes

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Abstract

Conventional and multifunctional cationic liposomes that efficiently deliver plasmid DNA (pDNA) were obtained. Partial inhibition of receptor-mediated endocytosis of pDNA complexes with multifunctional cationic liposomes containing folate lipids was shown in the presence of free folic acid in the cellular medium. Additional formation of pDNA complexes with the nuclear localization signal (NLS) peptide allowed increasing the efficiency of green fluorescent protein expression by 1.5–2 times using conventional and multifunctional cationic liposomes. Addition of the NLS peptide to pDNA and subsequent formation of complexes with cationic liposomes can be used to solve the problem of efficient pDNA delivery into eukaryotic cells.

About the authors

E. V Shmendel

Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University

Email: elena_shmendel@mail.ru
Moscow, Russia

O. V Markov

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences

Novosibirsk, Russia

M. A Zenkova

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences

Novosibirsk, Russia

M. A Maslov

Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University

Moscow, Russia

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