NLS Peptide Improves the Efficiency of pDNA Delivery into Eukaryotic Cells by Cationic Liposomes

Мұқаба

Дәйексөз келтіру

Толық мәтін

Ашық рұқсат Ашық рұқсат
Рұқсат жабық Рұқсат берілді
Рұқсат жабық Рұқсат ақылы немесе тек жазылушылар үшін

Аннотация

Conventional and multifunctional cationic liposomes that efficiently deliver plasmid DNA (pDNA) were obtained. Partial inhibition of receptor-mediated endocytosis of pDNA complexes with multifunctional cationic liposomes containing folate lipids was shown in the presence of free folic acid in the cellular medium. Additional formation of pDNA complexes with the nuclear localization signal (NLS) peptide allowed increasing the efficiency of green fluorescent protein expression by 1.5–2 times using conventional and multifunctional cationic liposomes. Addition of the NLS peptide to pDNA and subsequent formation of complexes with cationic liposomes can be used to solve the problem of efficient pDNA delivery into eukaryotic cells.

Авторлар туралы

E. Shmendel

Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University

Email: elena_shmendel@mail.ru
Moscow, Russia

O. Markov

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences

Novosibirsk, Russia

M. Zenkova

Institute of Chemical Biology and Fundamental Medicine, Siberian Branch, Russian Academy of Sciences

Novosibirsk, Russia

M. Maslov

Lomonosov Institute of Fine Chemical Technologies, MIREA – Russian Technological University

Moscow, Russia

Әдебиет тізімі

  1. Scalzo S., Santos A.K., Ferreira H.A.S., Costa P.A., Prazeres P.H.D.M., da Silva N.J.A., Guimarães L.C., E Silva M.M., Rodrigues Alves M.T.R., Viana C.T.R., Jesus I.C.G., Rodrigues A.P., Birbrair A., Lobo A.O., Frezard F., Mitchell M.J., Guatimosim S., Guimaraes P.P.G. // Int. J. Nanomedicine. 2022. V. 17. P. 2865–2881. https://doi.org/10.2147/IJN.S366962
  2. Prazeres P.H.D.M., Ferreira H., Costa P.A.C., da Silva W., Alves M.T., Padilla M., Thatte A., Santos A.K., Lobo A.O., Sabino A., Del Puerto H.L., Mitchell M.J., Guimaraes P.P.G. // Int. J. Nanomedicine. 2023. V. 18. P. 5891–5904. https://doi.org/10.2147/IJN.S424723
  3. Lu B., Lim J.M., Yu B., Song S., Neeli P., Sobhani N.K.P., Bonam S.R., Kurapati R., Zheng J., Chai D. // Front. Immunol. 2024. V. 15. P. 1–24. https://doi.org/10.3389/fimmu.2024.1332939
  4. Baghban R., Ghasemian A., Mahmoodi S. // Arch. Microbiol. 2023. V. 205. P. 1–15. https://doi.org/10.1007/s00203-023-03480-5
  5. Lim M., Badruddoza A.Z.M., Firdous J., Azad M., Mannan A., Al-Hilal T.A., Cho C.S., Islam M.A. // Pharmaceutics. 2020. V. 12. P. 1–29. https://doi.org/10.3390/pharmaceutics12010030
  6. Durymanov M., Reineke J. // Front. Pharmacol. 2018. V. 9. P. 1–15. https://doi.org/10.3389/fphar.2018.00971
  7. Amoako K., Mokhammad A., Malik A., Yesudasan S., Wheba A., Olagunju O., Gu S.X., Yarovinsky T., Faustino E.V.S., Nguyen J., Hwa J. // Front. Med. Technol. 2025. V. 7. P. 1591119. https://doi.org/10.3389/fmedt.2025.1591119.
  8. Xu E., Saltzman W.M., Piotrowski-Daspit A.S. // J. Control. Release. 2021. V. 335. P. 465–480. https://doi.org/10.1016/j.jconrel.2021.05.038
  9. Cheng X., Lee R.J. // Adv. Drug Deliv. Rev. 2016. V. 99. P. 129–137. https://doi.org/10.1016/j.addr.2016.01.022
  10. Kabilova T.O., Shmendel E.V., Gladkikh D.V., Chernolovskaya E.L., Markov O.V., Morozova N.G., Maslov M.A., Zenkova M.A. // Eur. J. Pharm. Biopharm. 2018. V. 123. P. 59–70. https://doi.org/10.1016/j.ejpb.2017.11.010
  11. Dilliard S.A., Siegwart D.J. // Nat. Rev. Mater. 2023. V. 8. P. 282–300. https://doi.org/10.1038/s41578-022-00529-7
  12. Lin D.H., Hoelz A. // Annu. Rev. Biochem. 2019. V. 88. P. 725–783. https://doi.org/10.1146/annurev-biochem-062917-011901
  13. Губанова Н.В., Морозова К.Н., Киселева Е.В. // Цитология. 2006. V. 11. P. 887–899.
  14. Roy S.M., Garg V., Sivaraman S.P., Barman S., Ghosh C., Bag P., Mohanasundaram P., Maji P.S., Basu A., Dirisala A., Ghosh S.K., Maitymit R. // J. Drug Deliv. Sci. Technol. 2023. V. 83. P. 104408. https://doi.org/10.1016/j.jddst.2023.104408
  15. Yao J., Fan Y., Li Y., Huang L. // J. Drug Target. 2013. V. 21. P. 926–939. https://doi.org/10.3109/1061186X.2013.830310
  16. Fontes M.R.M., Teh T., Kobe B. // J. Mol. Biol. 2000. V. 297. P. 1183–1194. https://doi.org/10.1006/jmbi.2000.3642
  17. Mashal M., Attia N., Maldonado I., Enríquez Rodríguez L., Gallego I., Puras G., Pedraz J.L. // Eur. J. Pharm. Biopharm. 2024. V. 201. P. 114385. https://doi.org/10.1016/j.ejpb.2024.114385
  18. Kurihara D., Akita H., Kudo A., Masuda T., Futaki S., Harashima H. // Biol. Pharm. Bull. 2009. V. 32. P. 1303–1306. https://doi.org/10.1248/bpb.32.1303
  19. Nematollahi M.H., Torkzadeh-Mahanai M., Pardakhty A., EbrahimiMeimand H.A., Asadikaram G. // Artif. Cells Nanomed. Biotechnol. 2018. V. 46. P. 1781–1791. https://doi.org/10.1080/21691401.2017.1392316
  20. Bishani A., Makarova D.M., Shmendel E.V., Maslov M.A., Sen’kova A.V., Savin I.A., Gladkikh D.V., Zenkova M.A., Chernolovskaya E.L. // Pharmaceutics. 2023. V. 15. P. 92184. https://doi.org/10.3390/pharmaceutics15092184
  21. Shmendel E.V., Bakhareva S.A., Makarova D.M., Chernikov I.V., Morozova N.G., Chernolovskaya E.L., Zenkova M.A., Maslov M.A. // Russ. J. Bioorg. Chem. 2020. V. 46. P. 1250–1260. https://doi.org/10.1134/S106816202006031X
  22. Mornet E., Carmoy N., Lainé C., Lemiègre L., Le Gall T., Laurent I., Marianowski R., Férec C., Lehn P., Benvegnu T., Montier T. // Int. J. Mol. Sci. 2013. V. 14. P. 1477–1501. https://doi.org/10.3390/ijms14011477
  23. Wang S., Lee R.J., Cauchon G., Gorensteint D.G., Lowt P.S. // Proc. Natl. Acad. Sci. USA. 1995. V. 92. P. 3318–3322
  24. Xu Z., Jin J., Siu L.K.S., Yao H., Sze J., Sun H., Kung H., Poon W.S., Ng S.S.M., Lin M.C. // Int. J. Pharm. 2012. V. 426. P. 182–192. https://doi.org/10.1016/j.ijpharm.2012.01.009
  25. Jones S.K., Sarkar A., Feldmann D.P., Hoffmann P., Merkel O.M. // Biomaterials. 2017. V. 138. P. 35–45. https://doi.org/10.1016/j.biomaterials.2017.05.034
  26. van der Aa M.A.E.M., Koning G.A., d’Oliveira C., Oosting R.S., Wilschut K.J., Hennink W.E., Crommelin D.J.A. // J. Gene Med. 2005. V. 7. P. 208–217. https://doi.org/10.1002/jgm.643

Қосымша файлдар

Қосымша файлдар
Әрекет
1. JATS XML
Жүктеу

© Russian Academy of Sciences, 2025