Methylation levels in the 5' region of the TBX20 gene in the ascending aorta change in opposite direction in atherosclerosis and aneurysm

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Abstract

We found hypomethylation of 5 CpG sites in the 5’region of TBX20 gene (7p14.2) in the tissues of atherosclerotic aortic plaque compared to dilated part of aorta in patients with ascending aortic aneurysm. Using GEO database, we found that the DNA methylation level in the chr7:35253926-35262250 region changes in opposite direction in aortic dissection and aortic atherosclerosis. The results suggest an alteration in epigenetic regulation both in aortic atherosclerosis andaortic aneurysm.

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About the authors

Yu. А. Koroleva

Tomsk National Research Medical Center, Russian Academy of Sciences

Author for correspondence.
Email: yuliya.koroleva@medgenetics.ru

Research Institute of Medical Genetics

Russian Federation, 634050, Tomsk

I. A. Goncharova

Tomsk National Research Medical Center, Russian Academy of Sciences

Email: yuliya.koroleva@medgenetics.ru

Research Institute of Medical Genetics

Russian Federation, 634050, Tomsk

A. А. Zarubin

Tomsk National Research Medical Center, Russian Academy of Sciences

Email: yuliya.koroleva@medgenetics.ru

Research Institute of Medical Genetics

Russian Federation, 634050, Tomsk

S. А. Shipulina

Tomsk National Research Medical Center, Russian Academy of Sciences

Email: yuliya.koroleva@medgenetics.ru

Research Institute of Medical Genetics

Russian Federation, 634050, Tomsk

A. A. Sleptsov

Tomsk National Research Medical Center, Russian Academy of Sciences

Email: yuliya.koroleva@medgenetics.ru

Research Institute of Medical Genetics

Russian Federation, 634050, Tomsk

D. S. Panfilov

Tomsk National Research Medical Center, Russian Academy of Sciences

Email: yuliya.koroleva@medgenetics.ru

Research Institute of Cardiology

Russian Federation, 634012, Tomsk

В. N. Kozlov

Tomsk National Research Medical Center, Russian Academy of Sciences

Email: yuliya.koroleva@medgenetics.ru

Research Institute of Cardiology

Russian Federation, 634012, Tomsk

M. S. Nazarenko

Tomsk National Research Medical Center, Russian Academy of Sciences

Email: yuliya.koroleva@medgenetics.ru

Research Institute of Medical Genetics

Russian Federation, 634050, Tomsk

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2. Fig. 1. Localization and epigenetic context of the studied 5' region of the TBX20 gene. Thin colored vertical lines mark five DMCs between the atherosclerotic plaque of the dilated part of the aorta not affected by atherosclerosis, the dilated part of the aorta and the non-dilated proximal part of the aorta (this study). Wide vertical stripes indicate: yellow – regions hypomethylated in myocardial tissues with tetrad Fallot [18, 19], blue – regions hypomethylated in aortic tissues with atherosclerosis [17]. The differences in the level of methylation between the unaffected aortic tissues and the atherosclerotically altered aorta for the three regions (from left to right) were 41, 29 and 30%. Red horizontal lines indicate regions of hypermethylated CpG sites in aortic dissection (GSE84274 [8]), blue horizontal lines indicate regions of hypomethylated CpG sites in aortic atherosclerosis compared to unaffected aortic tissue (GSE46394 [8]). All tracks are received and/or aligned on UCSC GenomeBrowser (GRCh38 genome assembly).

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