Seasonal dynamics of antioxidant enzyme activity and photooxidative processes in the dominant macroalgal species of the Sea of Japan

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Variations in the activity of the key antioxidant enzymes (superoxide dismutase, catalase, glutathione reductase, ascorbate peroxidase), as well as in the levels of lipid peroxidation (LP) and photosynthetic activity, were studied in five common species of macroalgae during their winter and summer vegetative season in the Sea of Japan. For most of the studied macroalgae, the highest intra-annual activities of antioxidant enzymes are observed during the colder months, while the lowest activities are observed during the warmer months. All the studied macroalgae display consistently high levels of photosynthesis during the period from December to February. A dramatic increase in the LP level and a suppression of photosynthetic activity are recorded during July and August for most of the studied macroalgae. The seasonal patterns of antioxidant enzyme activity and photooxidative processes in macroalgal tissues are discussed in terms of their regulation by fluctuations in illumination and seawater temperature in the habitats of the plants.

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Sobre autores

E. Belotsitsenko

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences

Autor responsável pela correspondência
Email: belotsitsenko_es@mail.ru
ORCID ID: 0009-0009-1852-8114
Rússia, Vladivostok 690041

I. Yakovleva

A.V. Zhirmunsky National Scientific Center of Marine Biology, Far Eastern Branch, Russian Academy of Sciences

Email: belotsitsenko_es@mail.ru
ORCID ID: 0000-0003-0299-6983
Rússia, Vladivostok 690041

Bibliografia

  1. Кравченко А.О., Белоциценко Е.С., Яковлева И.М. и др. Сезонные изменения содержания фотосинтетических пигментов у красной водоросли Ahnfeltiopsis flabelliformis Японского моря // Изв. ТИНРО. 2011. Т. 166. С. 123–133.
  2. Ли Б.Д. Разделение, идентификация и количественное определение фотосинтетических пигментов макробентосных водорослей // Экологические аспекты фотосинтеза морских макроводорослей. Владивосток: ДВНЦ АН СССР, 1978. С. 38–54.
  3. Шилина М.В., Мусатова О.В., Ивановский В.В. Биометрия: учебно-методический комплекс. Витебск: Изд-во Витеб. гос. ун-та им. П.М. Машерова, 2011. 182 с.
  4. Яковлева И.М., Белоциценко Е.С. Антиоксидантный потенциал макроводорослей Японского моря // Биол. моря. 2017. Т. 43. № 5. С. 372–382.
  5. Alscher R.G., Erturk N., Health L.S. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants // J. Exp. Bot. 2002. V. 53. P. 1331–1341. https://doi.org/10.1093/jexbot/53.372.1331
  6. Asada K. Production and scavenging of reactive oxygen species in chloroplasts and their functions // Plant Physiol. 2006. V. 141. P. 391–396. https://doi.org/10.1104/pp.106.082040
  7. Bischof K., Kräbs G., Wiencke C., Hanelt D. Solar ultraviolet radiation affects the activity of ribulose-1,5-bisphosphate carboxylase-oxygenase and the composition of photosynthetic and xanthophyll cycle pigments in the intertidal green alga Ulva lactuca L. // Planta. 2002. V. 215. P. 502–509. https://doi.org/10.1007/s00425-002-0774-9
  8. Bischof K., Rautenberger R. Seaweed responses to environmental stress: reactive oxygen and antioxidative strategies // Seaweed biology. Ecol. Stud. 2012. V. 219. P. 109–132.
  9. Cruces E., Flores-Molina M.R., Díaz M.J. et al. Phenolics as photoprotective mechanism against combined action of UV radiation and temperature in the red alga Gracilaria chilensis? // J. Appl. Phycol. 2018. V. 30. P. 1247–1257. https://doi.org/10.1007/s10811-017-1304-2
  10. Cruces E., Rautenberger R., Cubillos V.M. et al. Interaction of photoprotective and acclimation mechanisms in Ulva rigida (Chlorophyta) in response to diurnal changes in solar radiation in Southern Chile // J. Phycol. 2019. V. 55. P. 1011–1027. https://doi.org/10.1111/jpy.12894
  11. Galindo V., Levasseur M., Mundy C.J. et al. Contrasted sensitivity of DMSP production to high light exposure in two Arctic under-ice blooms // J. Exp. Mar. Biol. Ecol. 2016. V. 475. P. 38–48. https://doi.org/10.1016/j.jembe.2015.11.009
  12. Gambichler V., Zuccarello G.C., Karsten U. Seasonal changes in stress metabolites of native and introduced red algae in New Zealand // J. Appl. Phycol. 2021. V. 33. P. 1157–1170. https://doi.org/10.1007/s10811-020-02365-0
  13. Graiff A., Karsten U. Antioxidative properties of Baltic Sea keystone macroalgae (Fucus vesiculosus, Phaeophyceae) under ocean warming and acidification in a seasonally varying environment // Biology (Basel). 2021. V. 10. P. 13–30. https://doi.org/10.3390/biology10121330
  14. Granbom M., Pedersén M., Kadel P., Lüning K. Circadian rhythm of photosynthetic oxygen evolution in Kappaphycus alvarezii (Rhodophyta): dependence on light quantity and quality // J. Phycol. 2001. V. 37. P. 1020–1025.
  15. Heinrich S., Valentin K., Frickenhaus S., Wiencke C. Temperature and light interactively modulate gene expression in Saccharina latissima (Phaeophyceae) // J. Phycol. 2015. V. 51. № 1. P. 93–108. https://doi.org/10.1111/jpy.12255
  16. Jassby A.D., Platt T. Mathematical formulation of the relationship between photosynthesis and light for phytoplankton // Limnol. Oceanogr. 1976. V. 21. P. 540–547.
  17. Ji Y., Gao K. Effects of climate change factors on marine macroalgae: a review // Adv. Mar. Biol. 2021. V. 88. P. 91–136. https://doi.org/10.1016/bs.amb.2020.11.0
  18. Lalegerie F., Gager L., Stiger-Pouvreau V., Connan S. The stressful life of red and brown seaweeds on the temperate intertidal zone: effect of abiotic and biotic parameters on the physiology of macroalgae and content variability of particular metabolites // Adv. Bot. Res. 2020. V. 95. P. 247–287. https://doi.org/10.1016/bs.abr.2019.11.007
  19. Liu C., Zou D., Yang Y. et al. Temperature responses of pigment contents, chlorophyll fluorescence characteristics, and antioxidant defenses in Gracilariopsis lemaneiformis (Gracilariales, Rhodophyta) under different CO2 levels // J. Appl. Phycol. 2017. V. 29. P. 983–991. https://doi.org/10.1007/s10811-016-0971-8
  20. Lohrmann N.L., Logan B.A., Jonson A.S. Seasonal acclimatization of antioxidants and photosynthesis in Chondrus crispus and Mastocarpus stellatus, two co-occurring red algae with differing stress tolerances // Biol. Bull. 2004. V. 207. P. 225–232.
  21. Ma L.-H., Tian L., Wang Y-Q. et al. Physiological and transcriptome analysis of acclimatory response to cold stress in marine red alga Pyropia yezoensis // Algae. 2024. V. 39. № 1. P. 17–30. https://doi.org/10.4490/algae.2024.39.3.7
  22. Migné A., Duong G., Menu D. et al. Dynamics of Fucus serratus thallus photosynthesis and community primary production during emersion across seasons: canopy dampening and biochemical acclimation // Peer Community J. 2021. V. 1. Art. ID e32. https://doi.org/10.24072/pcjournal.42
  23. Rezayian M., Niknam V., Ebrahimzadeh H. Oxidative damage and antioxidative system in algae // Toxicol. Rep. 2019. V. 6. P. 1309–1313. https://doi.org/10.1016/j.toxrep.2019.10.001
  24. Sampath-Wiley P., Neefus C., Jahnke L.S. Seasonal effects of sun exposure and emersion on intertidal seaweed physiology: fluctuations in antioxidant contents, photosynthetic pigments and photosynthetic efficiency in the red algae Porphyra umbilicalis Kützing (Rhodophyta, Bangiales) // J. Exp. Mar. Biol. Ecol. 2008. V. 361. P. 83–91.
  25. Sen S. Antioxidative defense in plants in response to seasonal environmental stress // Asian J. Sci. Technol. 2020. V. 11. № 3. P. 10849–10862.
  26. Shakhmatova O., Ryzhik I. Seasonal dynamics of catalase activity in Cystoseira crinita (Black Sea) and Fucus vesiculosus (Barents Sea) // Ecol. Chem. Eng. Soc. 2020. V. 27. P. 643–650.
  27. Yang J.J., Yu D.C., Ma Y.F. et al. Antioxidative defense response of Ulva prolifera under high or low-temperature stimulus // Algal Res. 2019. V. 44. Art. ID 101703. https://doi.org/10.1016/j.algal.2019.101703
  28. Zhu S., Gu D., Lu C. et al. Cold stress tolerance of the intertidal red alga Neoporphyra haitanensis // BMC Plant Biol. 2022. V. 22. Art. ID 114. https://doi.org/10.1186/s12870-022-03507-x

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2. Fig. 1. Superoxide dismutase (SOD) activity in macroalgae from Sobol Bay (Peter the Great Bay, Sea of ​​Japan) during the winter and summer vegetation periods of 2005–2006 and 2010. Here and in Figs. 2 and 3, the mean arithmetic values ​​± SD are presented, n = 4–5; the same superscript letter indices indicate the absence of statistically significant intra-annual differences between the values ​​of the studied parameter in different months, at p < 0.05 (ANOVA, Turkey HSD test); uppercase letter indices are used for Ulva lactuca, Costaria costata and Devaleraea stenogona, lowercase – for Undaria pinnatifida and Tichocarpus crinitus.

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3. Fig. 2. Catalase (CAT) activity in macroalgae of Sobol Bay (Peter the Great Bay, Sea of ​​Japan) during the winter and summer vegetation periods of 2005–2006 and 2010.

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4. Fig. 3. Activity of glutathione reductase (GR, 2005–2006) and ascorbate peroxidase (APO, 2010) in macroalgae of Sobol Bay (Peter the Great Bay, Sea of ​​Japan) during the winter and summer vegetation periods.

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