Influence of the Cultivation Conditions of Glioblastoma Cells on the Expression of Transcription Factors Genes

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Abstract

Cellular heterogeneity is a feature of glioblastoma, a malignant and aggressive brain tumor, and one of the reasons for the ineffectiveness of standard treatment methods, probably arising from the differentiation of tumor stem cells. The causes of increased adaptive abilities and resistance of glioblastoma cells to drugs and stress arise at the level of molecular processes. For this reason, there were evaluated changes in the expression of the genes of transcription factors MYCN, MYCC, PHOX2A and PHOX2B, which are involved in the regulation of the cell cycle and differentiation, using real-time PCR in response to changes in cultivating conditions in this work. As a result, it was shown that primary cultures in an environment with fetal bovine serum acquire morphology and gene expression similar to cell lines. In addition, it was shown for the first time that all cell lines and primary cultures of glioblastoma differ in the expression profiles of the studied genes, however, in response to changes in cultivating conditions, all of them demonstrate a multiple increase in MYCN expression, as well as the opposite response of PHOX2A and PHOX2B, indicating a possible role of these genes in glioblastoma resistance to stress. The obtained data should be taken into account while selecting individual treatment for patients, as well as when developing therapeutic agents and further investigating molecular processes in glioblastoma cells.

About the authors

A. I. Rezekina

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Moscow, Russia

D. V. Mazur

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Moscow, Russia

M. I. Shakhparonov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences

Moscow, Russia

N. V. Antipova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry of the Russian Academy of Sciences; National Research University "Higher School of Economics"

Email: nadine.antipova@gmail.com
Moscow, Russia; Moscow, Russia

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