Proinflammatory Phenotype of CD161+ Double-Negative T Cells

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Abstract

Double-negative (DN) CD3+CD4-CD8- T lymphocytes represent a subgroup of unconventional peripheral T cells. Although their presence in peripheral blood is relatively low (3–5%), they play an important role in the development of inflammatory responses, oncological, autoimmune diseases, and allergic asthma. In the present study, we characterized the cytokine profile of CD3+CD4-CD8- DN T cells using multiplex technology with a wide panel of analytes. Our results demonstrate that the cytokine profile of proinflammatory αβTCR+CD161+ DN T cells includes the cytokines TNFα, IFNγ, IL-3, IL-13, and IL-27; the chemokines IL-8/CXCL8, MIG/CXCL9, MIP-1α/CCL3, MIP-1β/CCL4, and RANTES/CCL5; as well as the growth factors M-CSF and GM-CSF.

About the authors

I. V Bogdanov

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences

Moscow, Russia

T. V Ovchinnikova

Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences; Lomonosov Moscow State University, Faculty of Biology

Email: ovch@ibch.ru
Department of Bioorganic Chemistry Moscow, Russia

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