APPLICATION OF SENSING COMPOSITE FILMS “CROSS-LINKED POLYVINYL ALCOHOL—MAGNETITE” FOR THE DIGITAL COLORIMETRIC DETERMINATION OF GLUCOSE AND FRUCTOSE IN HONEY

A new method for the determination of water soluble carbohydrates (glucose and fructose) was developed with use of sensing composite films “cross-linked polyvinyl alcohol—magnetite”. It was proposed to apply the digital colorimetry method as a tool for the registration of film response in RGB space. The water-soluble carbohydrates determination was carried out in phosphate-borate buffer solution at pH value 8.5 and borax concentration 0.05 mol/dm3. It was performed that the most sensitive film was the one impregnated by iron salts solution during 2 minutes before precipitation of Fe₃O₄ particles. The LOD of glucose and fructose was equal to 11 mmol/dm3. The proposed approach to the determination of glucose and fructose, which does not require special laboratory equipment to measure the analytical response, can serve as an alternative to the photometric ferricyanide method for the determination of glucose and fructose.