Solid-state method of production of probiotic feed additives for farm animals

A technology has been developed for producing a probiotic preparation by solid-state cultivation of a mixed culture of Bacillus subtilis B-8130, B. subtilis B-2984, B. subtilis B-4099 on beet pulp, which is shown in two preparations (ProStor and GerbaStor). Solid-state fermentation was carried out under conditions of limited access to oxygen at a temperature of 40 ± 5°C, pH 7.5‒8.0 and mixture humidity 45 ± 3% for 48‒50 hours. During the process, acidification of the reaction mass was observed, a significant increase in cell titer and the formation of a biofilm on phytosorbent. The cell titer depended on the factor “stage of fermentation”, but did not depend on the factor type of preparation ‒ ProStor or GerbaStor. Among the identified volatile products, by 48 hours of fermentation the content of acetic acid, 3-methylbutanoic acid, p-guaiacol and 2-n-pentylfuran increased, and aromatic substances of the phenolic series decreased. Additional metabolites were obtained that enhance the antibacterial properties and taste of the probiotic preparation. The formation of a biofilm made it possible to preserve viable cells during drying (45°C): in ProStor 1.2 × 10⁸ CFU/g, and in GerbaStor 0.58 × 10⁸ CFU/g. In HerbaStore with an expanded range of medicinal plants, a decrease in the titer of bacilli is associated with dehydration during drying and with the antibacterial properties of phytocomponents.