Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65161710.31857/S0869813924070068BDQFTDEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleAnalysis of correlations between behavioral parameters in the elevated plus maze and the levels of interleukin-1beta in blood plasma in ratsАнализ корреляций между параметрами поведения в приподнятом крестообразном лабиринте и уровнем интерлейкина-1бета в плазме крови у крысKomyshevaN. P.КомышеваН. П.
Russian Federation
agarina@bionet.nsc.ruShishkinaG. T.ШишкинаГ. Т.
Russian Federation
agarina@bionet.nsc.ruMukhamadeevaA. I.МухамадееваА. И.
Russian Federation
agarina@bionet.nsc.ruDygaloN. N.ДыгалоН. Н.
Russian Federation
agarina@bionet.nsc.ruInstitute of Cytology and Genetics, Siberian Branch of Russian Academy of SciencesИнститут цитологии и генетики СО РАН1507202411071147115701022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://transsyst.ru/0869-8139/article/view/651617

Peripheral cytokines may influence psychoemotional behavior, but the role of interleukin-1beta (IL-1beta) in altering anxiety and motor activity in response to inflammatory activation remains unclear. To clarify this issue, correlations between behavioral parameters in the elevated plus maze (EPM) test and plasma levels of IL-1beta after administration of the proinflammatory stimulus lipopolysaccharide (LPS) in different modes were analyzed in adult male rats. LPS in doses of 0.5 or 5 mg/kg, as well as physiological solution (control), were administered to rats intraperitoneally. The most pronounced behavioral effect 24 hours after a single injection was an endotoxin dose-dependent inhibition of the animals’ motor activity. After a dose of 5 mg/kg, increased anxious behavior was also noted every other day. The behavioral changes caused by the high dose of endotoxin were completely normalized after a week. The behavior of the animals one day after the end of repeated injections of LPS at a lower dose for a week (0.5 mg/kg; once every two days) also did not differ from the control. The inhibition of motor activity after LPS could be due to an increase in the level of IL-1beta in the blood plasma, as indicated by the identified significant negative correlations between IL-1beta and the corresponding behavioral parameters. No significant correlation was found between the peripheral level of IL-1beta and such a classic indicator of anxiety as the percentage of entries into the open arms of the maze. In general, the obtained results allow us to conclude that IL-1beta is an undoubted participant in the mechanism of the transient inhibitory effect of LPS on motor activity.

Периферические цитокины могут влиять на психоэмоциональное поведение, однако роль интерлейкина-1бета (ИЛ-1бета) в изменении тревожности и двигательной активности в ответ на активацию воспаления остается неясной. С целью прояснения этого вопроса у взрослых самцов крыс были проанализированы корреляции между поведенческими параметрами в тесте приподнятого крестообразного лабиринта (ПКЛ) и уровнем ИЛ-1бета в плазме крови после введения в разных режимах провоспалительного стимула липополисахарида (ЛПС). ЛПС в дозах 0.5 или 5 мг/кг, а также физиологический раствор (контроль) вводили крысам внутрибрюшинно. Наиболее выраженным поведенческим эффектом через сутки после однократной инъекции было зависимое от дозы эндотоксина угнетение двигательной активности животных. После дозы 5 мг/кг через сутки также отмечалось повышенное тревожное поведение. Поведенческие изменения, вызванные высокой дозой эндотоксина, были полностью нормализованы через неделю. Поведение животных через сутки после окончания повторных инъекций ЛПС в меньшей дозе в течение недели (0.5 мг/кг; 1 раз в два дня) также не отличалось от контроля. Угнетение двигательной активности после ЛПС могло быть обусловлено увеличением уровня ИЛ-1бета в плазме крови, на что указывают выявленные достоверные отрицательные корреляции между содержанием ИЛ-1бета и соответствующими поведенческими параметрами. Между периферическим уровнем ИЛ-1бета и таким классическим показателем тревожности, как процент входов в открытые рукава лабиринта, достоверной корреляции не обнаружено. В целом полученные результаты позволяют заключить, что ИЛ-1бета является несомненным участником механизма транзиторного угнетающего влияния ЛПС на двигательную активность.

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