Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65161610.31857/S0869813924070057BDUWVCEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleAlterations in tissue content of iron and zinc in mice bearing hepatoma 22a and their correction by zinc sulphate supplementationНарушения содержания железа и цинка в тканях у мышей при росте гепатомы 22а и их коррекция с помощью сульфата цинкаZelenskyiЕ. А.ЗеленскийЕ. А.
Russian Federation
ekissele@yandex.ruRuttoK. V.РуттоК. В.
Russian Federation
ekissele@yandex.ruTrulioffA. S.ТрулевА. С.
Russian Federation
ekissele@yandex.ruMagazenkovaD. N.МагазенковаД. Н.
Russian Federation
ekissele@yandex.ruSokolovA. V.СоколовА. В.
Russian Federation
ekissele@yandex.ruKisselevaЕ. P.КиселеваЕ. П.
Russian Federation
ekissele@yandex.ruInstitute of Experimental MedicineИнститут экспериментальной медициныNorth-Western Medical University named after II MechnikovСеверо-Западный государственный медицинский университет им. И. И. Мечникова МЗ РФ1507202411071128114601022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://transsyst.ru/0869-8139/article/view/651616

It is known that many tumors induce iron and zinc deficiency in the organism. We studied the content of these metals, as well as the specific activity of two antioxidant metal-dependent enzymes – catalase and superoxide dismutase of three distal organs (thymus, liver and spleen) in animals bearing transplantable hepatoma 22a. These alterations were compared to weight changes of organs. On day 21 of tumor growth, as compared to control group, nonheme iron content in all three organs was decreased, and zinc content – only in the thymus. The specific activities of catalase and superoxide dismutase were both increased in the thymus, while in the liver activity of superoxide dismutase decreased. At the same time point thymic involution and splenomegaly were developed. In order to normalize metal content mice bearing hepatoma 22a were supplemented with 22 mkg of zinc sulphate per ml of drinking water during 3 weeks. Zinc sulphate supplementation partly compensated zinc deficiency in the thymus, increased zinc content in the liver and restored iron content in three organs. It also normalized superoxide dismutase activity in the liver and had no influence on enzymes in other organs. Zinc supplementation did not influence the weight of spleen and liver, but prevented the development of thymic involution. Moreover, metal deficiency in the thymus was restored while the activity of antioxidant enzymes remained unchanged. Based on this we can conclude that thymus involution in hepatoma 22a mice was associated with iron and zinc deficiency in this organ and was not linked with antioxidant enzyme activity, while splenomegaly had no relation to both types of parameters in the spleen. Thus, zinc sulphate positively influences metabolism of two vital trace elements – zinc and iron in animals bearing hepatoma 22a, what contributes to maintaining of the central immune organ – the thymus, and along with this it improves antioxidant system of the liver.

Известно, что рост многих опухолей приводит к развитию дефицита железа и цинка в организме. Исследовали содержание этих металлов, а также удельную активность двух антиоксидантных металлоферментов – каталазы и супероксиддисмутазы в трех удаленных от опухоли органах (тимусе, печени и селезенке) при росте перевиваемой гепатомы 22а. Выявленные сдвиги сопоставляли с изменениями массы органов. На 21-е сутки опухолевого роста содержание негемового железа было снижено по сравнению с контролем во всех трех органах, а цинка – только в тимусе. Специфические активности каталазы и супероксиддисмутазы были повышены в тимусе, в то время как в печени активность супероксиддисмутазы снижена. На этом же сроке наблюдали развитие инволюции тимуса и спленомегалии. Для нормализации содержания металлов мыши с гепатомой 22а получали дополнительно 22 мкг сульфата цинка на мл питьевой воды в течение трех недель. Прием сульфата цинка частично компенсировал дефицит цинка в тимусе, повышал его содержание в печени и восстанавливал уровень железа в трех органах. Он также нормализовал активность супероксиддисмутазы в печени, но не влиял на ферменты в других органах. Прием цинка не влиял на массу селезенки и печени, но тормозил развитие инволюции тимуса. При этом в тимусе восстанавливался дефицит микроэлементов, а активность антиоксидантных ферментов не менялась. На основании этого можно заключить, что инволюция тимуса при росте гепатомы 22а связана с дефицитом железа и цинка и не связана с активностью антиоксидантных ферментов в этом органе, а спленомегалия не ассоциирована ни с тем, ни с другим в селезенке. Таким образом, сульфат цинка оказывает позитивное действие на метаболизм двух важнейших микроэлементов – цинка и железа в организме животных с гепатомой 22а, что способствует сохранению центрального органа иммунной системы – тимуса, а также положительно влияет на антиоксидантную систему печени.

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