Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65158210.31857/S0869813923040039VIWGPCEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch of the Expression of IL-13 Receptors and IL-13R Genes in the Bronch of Rats with Ovalbumin-Induced Bronchial AsthmaИсследование экспрессии рецепторов к IL-13, генов IL-13R в бронхах крыс с овальбумин-индуцированной бронхиальной астмойBlazhevichL. E.БлажевичЛ. Е.lyu15041988@yandex.ruSmirnovaO. E.СмирноваО. Е.lyu15041988@yandex.ruKirilinaV. M.КирилинаВ. М.lyu15041988@yandex.ruMaslyukovP. M.МаслюковП. М.lyu15041988@yandex.ruPetrozavodsk State UniversityПетрозаводский государственный университет, Республика КарелияYaroslavl State Medical University of the Ministry of Healthcare of the Russian FederationЯрославский государственный медицинский университет Министерства здравоохранения Российской Федерации01042023109453054401022025Copyright ©; 2023, Л.Е. Блажевич, О.Е. Смирнова, В.М. Кирилина, П.М. МаслюковCopyright ©; 2023, Л.Е. Блажевич, О.Е. Смирнова, В.М. Кирилина, П.М. Маслюков2023Л.Е. Блажевич, О.Е. Смирнова, В.М. Кирилина, П.М. МаслюковЛ.Е. Блажевич, О.Е. Смирнова, В.М. Кирилина, П.М. Маслюковhttps://transsyst.ru/0869-8139/article/view/651582

This article presents the results of studies of the levels of expression of the IL13R gene and the levels of expression of the molecular IL13R in the bronchi of control rats and rats with ovalbumin-induced asthma. The choice of these receptors is due to the great importance of these structures in the pathogenesis of allergic bronchial asthma. Biological materials of 64 Wistar rats were studied using real-time PCR and immunohistochemical methods. As a result of the studies, it was found that under conditions of asthma development in the tissues of the bronchi of rats, a pronounced expression of the IL13R genes, a significant expression of the molecular receptor for interleukin-13, occurs. In bronchi with an intramural ganglion (bifurcation zone), the level of IL13R gene expression and the level of expression of the IL13R molecular receptor were significantly higher than in tracheal samples without ganglia. Sodium cromoglycate, administered to rats 5 hours after the last inhalation of ovalbumin, led to a decrease in the mRNA content in the bronchi of animals compared to animals that did not receive mast cell stabilizer treatment.

В статье представлены результаты исследований уровней экспрессии гена рецепторов интерлейкина-13 (IL-13R) и уровней экспрессии IL-13R в бронхах контрольных крыс и крыс с овальбумин-индуцированной астмой. Выбор этих рецепторов обусловлен большим значением данных структур в патогенезе аллергической бронхиальной астмы. Были исследованы биологические материалы 64 крыс линии Вистар при помощи метода ПЦР в режиме реального времени и иммуногистохимическим методом. Установлено, что в условиях развития астмы в тканях бронхов крыс происходит выраженная экспрессия генов IL13R, значительная экспрессия рецептора к IL-13. В участках бронхов, содержащих интрамуральные ганглии (область бифуркации), уровень экспрессии генов IL13R и уровень экспрессии рецептора IL-13R были значительно выше, чем в образцах трахеи без ганглиев. Кромогликат натрия, введенный в организм крыс через 5 ч после последней ингаляции овальбумином, приводил к снижению содержания мРНК в бронхах животных по сравнению с животными, не получавшими обработку стабилизатором тучных клеток.

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