Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65158110.31857/S086981392304009XVJOVIZEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИRole of L-Type Calcium Channels in the Increased Fatigue of Rat Soleus Muscle under Functional UnloadingРоль кальциевых каналов L-типа в повышенной утомляемости камбаловидных мышц крыс на фоне функциональной разгрузкиSharloK. A.ШарлоК. А.sharlokris@gmail.comLvovaI. D.ЛьвоваИ. Д.sharlokris@gmail.comTyganovS. A.ТыгановС. А.sharlokris@gmail.comSidorenkoD. A.СидоренкоД. А.sharlokris@gmail.comShenkmanB. S.ШенкманБ. С.sharlokris@gmail.comInstitute of Biomedical Problems of the Russian Academy of Sciences RASИнститут медико-биологических проблем Российской академии наук01042023109451752901022025Copyright ©; 2023, К.А. Шарло, И.Д. Львова, С.А. Тыганов, Д.А. Сидоренко, Б.С. ШенкманCopyright ©; 2023, К.А. Шарло, И.Д. Львова, С.А. Тыганов, Д.А. Сидоренко, Б.С. Шенкман2023К.А. Шарло, И.Д. Львова, С.А. Тыганов, Д.А. Сидоренко, Б.С. ШенкманК.А. Шарло, И.Д. Львова, С.А. Тыганов, Д.А. Сидоренко, Б.С. Шенкманhttps://transsyst.ru/0869-8139/article/view/651581

Excessive long-term accumulation of calcium ions in the myoplasm of skeletal muscles can negatively affect mitochondria and lead to muscle dysfunction. The aim of our study was to identify the role of L-type calcium channels in the development of increased fatigue rat soleus muscle under functional unloading. Young male Wistar rats were divided into three groups of 8 animals each: the vivarium control group (C), the group subjected to hind limb unloading for 7 days (7HS) and the group with 7 days of hindlimb unloading with daily intraperitoneal injections of nifedipine (7 mg/kg body weight). The administration of nifidipine during hindlimb unloading prevented the upregulation of calcium-dependent phosphorylation of calcium-calmodulin kinase II (CaMK II), prevented the increase in fatigue and contributed to the preservation of mitochondrial proteins, DNA and mRNA expression of a number of genes that regulate mitochondrial biogenesis.

Избыточное долговременное накопление ионов кальция в миоплазме скелетных мышц может негативно влиять на митохондрии и приводить к нарушению функционирования мышцы. Целью настоящего исследования стало выявление роли кальциевых каналов L-типа в развитии повышенной утомляемости камбаловидной мышцы крыс в условиях функциональной разгрузки. Cамцы крыс Вистар были разделены на три группы по 8 животных в каждой: группа виварного контроля (С), группа, подвергнутая разгрузке задних конечностей на протяжении 7 сут (7HS), и группа, подвергнутая 7-суточной разгрузке задних конечностей с ежедневным внутрибрюшинным введением нифедипина (7 мг/кг массы тела). Введение нифедипина на фоне вывешивания предотвратило рост кальций-зависимого фосфорилирования кальций-кальмодулин киназы II и повышение утомляемости и способствовало сохранению митохондриальных белков, ДНК и экспрессии мРНК ряда генов-регуляторов биогенеза митохондрий в камбаловидных мышцах животных.

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