Russian Journal of PhysiologyRussian Journal of PhysiologyРоссийский физиологический журнал им. И.М. Сеченова0869-81392658-655XThe Russian Academy of Sciences65153910.31857/S0869813923080046NOYLJTEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИThe Effects of Social Hierarchy Establishment in Resident–Intruder Model on Testicular Function in Laboratory Mice of Different Inbred StrainsВлияние формирования доминантно-субординантых отношений в модели резидент–интрудер на тестикулярную функцию у лабораторных мышей инбредных линий PT и CBA/LacKleshchevM. A.КлещевМ. А.max82cll@bionet.nsc.ruOsadchukA. V.ОсадчукА. В.max82cll@bionet.nsc.ruOsadchukL. V.ОсадчукЛ. В.max82cll@bionet.nsc.ruInstitute of Cytology and Genetics, Siberian Branch of Russian Academy of SciencesФедеральный исследовательский центр Институт цитологии и генетики СО РАН0108202310981108112301022025Copyright ©; 2023, М.А. Клещев, А.В. Осадчук, Л.В. ОсадчукCopyright ©; 2023, М.А. Клещев, А.В. Осадчук, Л.В. Осадчук2023М.А. Клещев, А.В. Осадчук, Л.В. ОсадчукМ.А. Клещев, А.В. Осадчук, Л.В. Осадчукhttps://transsyst.ru/0869-8139/article/view/651539

Male reproductive success is known to be related with ability to social dominance and number and quality of spermatozoa as well as the production of reproductive hormones. The relationships between social dominance and testicular function are affected by genotype and environmental conditions of social hierarchy formation, and male territorial status (resident or intruder). However, the mechanisms of these relationships are still poorly understood. The aim of this study was to investigate effects of genotype and of familiarization with the habitat on testicular function during social hierarchy formation in experimental model of social hierarchy in laboratory mice using resident-intruder paradigm. Male of certain inbred strain (PT or CBA/Lac strain) was placed to experimental cage where male (resident) of other inbred strain (PT or CBA/Lac strain) has been living two days with female of DD/He strain. Social rank for each male was assessed after social hierarchy formation. Then testosterone level in serum and testes and epydidymal sperm quality were estimated after five days after the group formation. It was shown that social hierarchy formation did not affect testosterone level in serum and testes as well as percentage of sperm heads with abnormal morphology. However, the establishment of social hierarchy resulted in decreased sperm number in dominant-intruders of PT strains as well as decreased progressive sperm motility in subordinants of CBA/Lac strain regardless territorial status. To conclude, social hierarchy formation in experimental model of social hierarchy in laboratory mice affects epididymal sperm quality already five days after group formation, with pattern of these relationships depending on genotype, social rank and territorial status.

Известно, что репродуктивный успех самца зависит от способности к социальному доминированию, а также качества, количества сперматозоидов и адекватной продукции репродуктивных гормонов. Взаимосвязь между социальным доминированием и тестикулярной функцией может модулироваться генотипом и средовыми условиями формирования иерархии, в частности территориальным статусом самца (резидент или интрудер), однако характер и механизмы этой взаимосвязи недостаточно исследованы. Целью настоящей работы стало исследование влияния предварительного освоения территории и генотипа на тестикулярную функцию при установлении доминантно-субординантных отношений в экспериментальной модели социального доминирования у лабораторных мышей, используя парадигму “резидент–интрудер”. Для этого взрослому самцу-резиденту определенного генотипа (PT или CBA/Lac), который проживал с самкой линии DD/He двое суток в экспериментальной клетке, подсаживали самца-интрудера другой линии (PT или CBA/Lac) и оценивали социальный статус самцов после установления иерархических отношений. Через 5 дней после формирования группы у самцов оценивали уровень тестостерона в сыворотке и содержание его в семенниках, а также сперматогенные параметры. Установлено, что формирование иерархических отношений не оказывает влияния на уровень тестостерона в крови и семенниках и долю морфологически аномальных головок сперматозоидов у самцов мышей обеих линий, но приводит к уменьшению количества эпидидимальных сперматозоидов у доминантов-интрудеров линии РТ по сравнению с субординантами-интрудерами этой линии, а также снижению подвижности сперматозоидов у подчиненных самцов линии CBA/Lac независимо от территориального статуса. Таким образом, установление доминантно-субординантных отношений в этологической модели социальной иерархии у лабораторных мышей оказывает влияние на сперматогенную функцию самцов уже через 5 дней после формирования группы, при этом характер этого влияния зависит от генотипа животного, его социального и территориального статуса (резидент или интрудер).

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