Human PhysiologyHuman PhysiologyФизиология человека0131-16463034-6150The Russian Academy of Sciences68531010.31857/S0131164625010068VMSZVSArticlesСтатьиResearch ArticleDetermination of proteomic markers in dry spots of blood involved in the adaptation of the cardiovascular system in long-term space flights. Part I.Определение протеомных маркеров в сухих пятнах крови, включенных в адаптацию сердечно-сосудистой системы в длительных космических полетах. Часть IPastushkovaL. Kh.ПастушковаЛ. Х.
Russian Federation
daryakudryavtseva@mail.ruGoncharovaA. G.ГончароваА. Г.
Russian Federation
daryakudryavtseva@mail.ruKashirinaD. N.КаширинаД. Н.
Russian Federation
daryakudryavtseva@mail.ruLarinaI. M.ЛаринаИ. М.
Russian Federation
daryakudryavtseva@mail.ruInstitute of Biomedical Problems, RASФГБУН ГНЦ РФ – Институт медико-биологических проблем РАН15022025511637519062025Copyright ©; 2025, Russian Academy of SciencesCopyright ©; 2025, Российская академия наук2025Russian Academy of SciencesРоссийская академия наукhttps://transsyst.ru/0131-1646/article/view/685310

The complex of extreme factors of space flight induces various changes in the cardiovascular system at the structural and functional level. The study of protein markers by proteomics methods, included in the compensation of dysfunctional disorders of the cardiovascular system in long-term space flights, is relevant. The aim of the work: search for the main proteomic markers in dry spots of blood, included in the adaptation of the cardiovascular system during long-term space flights. To analyze the content of peptides in dry spot extracts, targeted quantitative chromatography-mass spectrometry with multiple reaction monitoring (LC-MRM MS) was used, using synthetic labeled standards (SIS). As a result of statistical and bioinformatics analysis, using ANDvisio software, it was established that the content of proteins involved in the adaptation of the cardiovascular system in dried blood spot extracts changed significantly during space flight: on the 7th day – 11 proteins, after 3 months – 5, after 6 months – 3 proteins. The main biological functions of these proteins are described, as applied to the duration of space flight and their participation in the adaptation of the cardiovascular system to a complex of extreme factors, including endothelial regeneration, angiogenesis and processes aimed at restoring the elasticity and contractility of vascular smooth muscle cells. Proteins involved in the control of cellular oxidative stress are expressed at all points in the study. The data obtained are of practical importance in relation to the assessment of the risks of cardiovascular events during long-term space flights.

Комплекс экстремальных факторов космического полета (КП) индуцирует различные изменения сердечно-сосудистой системы (ССС) на структурном и функциональном уровне. Актуально исследование белковых маркеров методами протеомики, включенных в компенсацию дисфункциональных нарушений ССС в длительных КП. Цель работы – поиск основных протеомных маркеров в сухих пятнах крови, включенных в адаптацию ССС во время длительных КП. Для анализа содержания пептидов в экстрактах сухих пятен применяли таргетный количественный хромато-масс-спектрометрический анализ с мониторингом множественных реакций (LC-MRM MS), с использованием синтетических меченых стандартов (SIS). В результате статистического и биоинформационного анализа, с использованием программного обеспечения ANDvisio, установлено, что в КП достоверно изменилось содержание в экстрактах сухих пятен крови белков, включенных в адаптацию ССС: на 7 сут –11 белков, через 3 мес. – 5 белков, через 6 мес. – 3 белка. Описаны основные биологические функции данных белков применительно к срокам КП и их участия в адаптации ССС к комплексу экстремальных факторов, в том числе регенерации эндотелия, ангиогенезу и процессам, направленным на восстановление эластичности и сократительной способности гладкомышечных клеток сосудов. Во всех точках исследования экспрессируются белки, участвующие в контроле клеточного окислительного стресса. Полученные данные имеют практическое значение применительно к оценке рисков сердечно-сосудистых событий во время длительных КП.

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