Journal of Evolutionary Biochemistry and PhysiologyJournal of Evolutionary Biochemistry and PhysiologyЖурнал эволюционной биохимии и физиологии0044-45293034-5529The Russian Academy of Sciences64810310.31857/S0044452924030082YWXRTYEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleA comparative distribution of parvalbumin-immunopositive neural elements in the spinal cord of newborn and adult catsОсобенности распределения системы парвальбумин-иммунопозитивных элементов в спинном мозге новорождённых кошекVeshchitskiiA. A.ВещицкийА. А.
Russian Federation
merkulyevan@infran.ruBelyaevA. V.БеляевА. В.
Russian Federation
merkulyevan@infran.ruMerkulyevaN. S.МеркульеваН. С.
Russian Federation
merkulyevan@infran.ruPavlov Institute of Physiology of the Russian Academy of SciencesИнститут физиологии им. И.П. Павлова РАН1505202460329930728012025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://transsyst.ru/0044-4529/article/view/648103

We analyzed characteristic features of the parvalbumin-immunostaining within the lumbosacral spinal cord of newborn kittens. In contrast to the adults, parvalbumin-immunostaining was mainly revealed for the sensory fibers located within the dorsal horns and in the medial part of the intermediate gray matter. The location of these fibers partially resembles the location of Clarke's nuclei, but lasted throughout the total length of the lumbar spinal cord and merged with the presumptive Stilling’s nuclei in the sacral region. Therefore, in newborns, in contrast to adults, the parvalbumin-immunostaining proprioceptive fibers seem like a single unit. We propose that with maturation, this system is restructured because of the spread of the neuronal and neuropil elements of the lumbar enlargement responsible for the locomotor control. As a result, two local nuclear complexes: Clarke’s and Stilling’s are retained. A single population of parvalbumin-immunostaining neurons in newborns are premotor interneurons located around the lamina IX. These neurons are characterized by the low or absent NeuN-immunostaining. We believe that this neurochemical feature may be inherent for these cells.

Проведено исследование особенностей экспрессии кальций-связывающего белка парвальбумина в пояснично-крестцовом отделе спинного мозга новорождённых и взрослых кошек. В отличие от взрослых животных, у новорожденных иммуномечение к парвальбумину выявлено главным образом в афферентных волокнах, расположенных в дорзальных рогах и медиальной части промежуточного серого вещества. Локализация этих волокон частично повторяет положение ядер Кларка, но не ограничена их классическими границами, охватывая всю протяжённость поясничного отдела и переходя в предполагаемое ядро Штиллинга, расположенное в крестцовом отделе. Таким образом, парвальбумин-иммунопозитивные проприоцептивные волокна у новорожденных, в отличие от взрослых, представляют собой единую систему. Полагаем, что с возрастом в связи с созреванием и разрастанием элементов поясничного утолщения, связанных, в первую очередь, с локомоторной функцией, происходит перестройка этой непрерывной системы волокон с выделением локальных элементов, таких как ядра Кларка и Штиллинга. Единственными спинальными нейронами, маркированными парвальбумином у новорожденных, являются премоторные интернейроны, расположенные вдоль курватуры пластины IX. Для этих клеток характерно полное или частичное отсутствие экспрессии нейронального белка NeuN, что свидетельствует об особенностях нейрохимического статуса таких нейронов.

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