Journal of Evolutionary Biochemistry and PhysiologyJournal of Evolutionary Biochemistry and PhysiologyЖурнал эволюционной биохимии и физиологии0044-45293034-5529The Russian Academy of Sciences64809310.31857/S0044452924030069YXGPSWEXPERIMENTAL ARTICLESЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИResearch ArticleStimulating effect of low concentrations of Eu3+ on spontaneous cardiac contractionsСтимулирующее влияние низких концентраций Eu3+ на спонтанные сердечные сокращенияSobolK. V.СобольК. В.
Russian Federation
peep9@yandex.ruKorotkovS. M.КоротковС. М.
Russian Federation
peep9@yandex.ruShemarovaI. V.ШемароваИ. В.
Russian Federation
peep9@yandex.ruNesterovV. P.НестеровВ. П.
Russian Federation
peep9@yandex.ruSechenov Institute of Evolutionary Physiology and Biochemistry Russian Academy of SciencesИнститут эволюционной физиологии и биохимии им. И.М. Сеченова Российской академии наук1505202460328229028012025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://transsyst.ru/0044-4529/article/view/648093

The negative cumulative effects of lanthanides on the human body are well known; they are associated mainly with the toxic effects of rare earth metals (REE) on muscle tissue. However, the effects of low concentrations of these metals on muscle are less understood. In our work, we found out an unusual stimulating effect of low concentrations of europium (Eu3+) on spontaneous contractions of atria of a frog. The purpose of this study was to study the stimulating effect of Eu3+ on the contraction of atria, both normally and in the presence of the mitochondrial respiration inhibitor sodium azide (NaN3). The study was carried out using two experimental models: muscle preparations obtained from isolated atria of the heart of the frog Rana ridibunda and mitochondria isolated from the heart of male Wistar rats. As a result of the studies, it was established that Eu3+ in a concentration of 0.2 mM, at a temperature of 20°C, potentiated contractions of the frog atria in situ; both the amplitude and the maximum rate of increase of single spontaneous contractions increased. Spontaneous atrial contractions became more resistant to the effects of 1 mM NaN3. At the same time, Eu3+ did not affect the respiration of energized mitochondria (activated by ADP (state 3) or 2,4-dinitrophenol (state 3UDNP). The intensity of this respiration decreased after the calcium load of mitochondria, regardless of the presence of Eu3+ in the medium. Thus, Eu3+ ions at low concentrations (0.2 mM) stimulated atrial contraction and had a positive inotropic effect. The stimulating effect of low concentrations of Eu3+ on the heart can be explained by the synergism in the action of Ca2+ and Eu3+ on calcium channels, stimulation of Ca2+-dependent processes in cardiomyocytes and the absence of a negative effect on mitochondrial respiration.

Негативные кумулятивные эффекты влияния лантаноидов на организм человека хорошо известны, они связаны, преимущественно, с токсическим воздействием редкоземельных металлов (РЗЭ) на мышечную ткань. Однако эффекты низких концентраций этих металлов на мышцы изучены значительно хуже. В своей работе мы выявили необычный стимулирующий эффект низкой концентрации европия (Eu3+) на спонтанные сокращения препаратов предсердий сердечной мышцы лягушки. Целью настоящего исследования было изучение стимулирующего влияния европия (Eu3+) на сокращение препаратов предсердий сердечной мышцы как в норме, так и в присутствии ингибитора митохондриального дыхания азида натрия (NaN3). Исследование проводилось с использованием двух экспериментальных моделей: мышечных препаратов, полученных из изолированных предсердий сердца лягушки Rana ridibunda, и митохондрий, выделенных из сердца самцов крыс линии Вистар. В результате проведенных исследований установлено, что Eu3+ в концентрации 0.2 мМ при температуре 20°С потенциировал сокращения предсердий лягушки in situ, при этом увеличивалась как амплитуда, так и максимальная скорость нарастания силы одиночных спонтанных сокращений. Спонтанные сокращения предсердий становились более устойчивыми к воздействию 1мМ NaN3. При этом Eu3+ не влиял на дыхание энергизованных митохондрий (активированное АДФ (состояние 3) или 2,4-динитрофенолом (состояние 3РДНФ)). Интенсивность этого дыхания снижалась в условиях кальциевой нагрузки митохондрий независимо от наличия в среде Eu3+. Таким образом, на основании проведенных исследований был сделан вывод о том, что ионы Eu3+ при низкой концентрации (0.2 мМ) стимулируют сокращение предсердий и оказывают положительное инотропное действие. Стимулирующий эффект низкой концентрации Eu3+ на сердце можно объяснить синергизмом в действии Са2+ и Eu3+ на кальциевые каналы стимуляцией Са2+-зависимых процессов в кардиомиоцитах и отсутствием негативного эффекта на дыхание митохондрий.

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