Cell and Tissue BiologyCell and Tissue BiologyЦитология0041-37713034-6061The Russian Academy of Sciences66959910.31857/S0041377124030101PDYRCMArticlesСтатьиResearch ArticleMethods for Image Analysis of Intracellular Structures of Actin Labeled with PhalloidinМетоды анализа изображений внутриклеточных структур актина, меченного фаллоидиномRevittserA. V.РевитцерА. В.
Russian Federation
eetytnet@gmail.comNegulyaevY. A.НегуляевЮ. А.
Russian Federation
eetytnet@gmail.comInstitute of Cytology Russian Academy of SciencesИнститут цитологии РАН1505202466329930627022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://transsyst.ru/0041-3771/article/view/669599

A cell is a complex three-dimensional system, which possesses a number of highly dynamic structures with extended, rugged, and uneven morphology. The actin cytoskeleton consists of fibrillar and globular actin, as well as auxiliary proteins that regulate organization. The shape and the rearrangements of actin cytoskeleton are closely related to functioning of the cell. The ability to characterize these changes allows scientists to confirm or refute any hypotheses in the research. Obtaining a numerical equivalent of the actin cytoskeleton organization could help compare actin structures in biological experiments (example: exposure to biologically active substances). The review summarizes methods for analyzing images of intracellular actin structures labeled with phalloidin using ImageJ. The methods considered make it possible to obtain a quantitative characteristic of the organization of actin structures for further evaluation and comparison of experimental results.

Клетка — это сложно организованная трехмерная система, обладающая целым рядом высокодинамичных структур, морфология которых сложна для описания, так как является протяженной, изрезанной, неровной. Актиновый цитоскелет, состоящий из фибриллярного и глобулярного актина, а также вспомогательных белков, регулирующих его организацию, является одной из таких структур. Изучение его организации и динамики — актуальный вопрос современной цитологии. Форма и перестройки актинового цитоскелета тесно связаны с функционированием клетки. Возможность какой-либо (качественной или количественной) характеристики изменений актинового цитоскелета позволяет подтверждать или опровергать гипотезы в ходе исследований, а также делать новые предположения. Количественная характеристика помогает объективно и непредвзято сравнивать актиновые структуры в различных экспериментах, выявляя воздействия биологических, химических и физических стимулов. В настоящее время для численной оценки актиновых структур клетки существует несколько доступных и легко реализуемых подходов с помощью бесплатного программного продукта ImageJ. В представленном обзоре обобщены методы анализа изображений внутриклеточных структур актина, меченных фаллоидином, с помощью ImageJ. Рассмотренные методы позволяют получать количественную характеристику организации актиновых структур для дальнейшей оценки и сравнения результатов экспериментов.

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