Russian Journal of GeneticsRussian Journal of GeneticsГенетика0016-67583034-5103The Russian Academy of Sciences68220010.31857/S0016675825040043UBJTVMГЕНЕТИКА ЖИВОТНЫХResearch ArticleWhole genome RNA sequencing of spermatozoa from holstein bulls with various cryoresistanceПолногеномное РНК-секвенирование сперматозоидов быков голштинской породы с различной криорезистентностьюBarkovaO. Yu.БарковаО. Ю.
Russian Federation

All-Russian Research Institute of Genetics and Breeding of Farm Animals (VNIIGRZh)

Всероссийский научно-исследовательский институт генетики и разведения сельскохозяйственных животных

barkoffws@list.ruStarikovaD. A.СтариковаД. А.
Russian Federation

All-Russian Research Institute of Genetics and Breeding of Farm Animals (VNIIGRZh)

Всероссийский научно-исследовательский институт генетики и разведения сельскохозяйственных животных

barkoffws@list.ruChistyakovaI. V.ЧистяковаИ. В.
Russian Federation

All-Russian Research Institute of Genetics and Breeding of Farm Animals (VNIIGRZh)

Всероссийский научно-исследовательский институт генетики и разведения сельскохозяйственных животных

barkoffws@list.ruPleshanovN. V.ПлешановН. В.
Russian Federation

All-Russian Research Institute of Genetics and Breeding of Farm Animals (VNIIGRZh)

Всероссийский научно-исследовательский институт генетики и разведения сельскохозяйственных животных

barkoffws@list.ruL.K. Ernst Federal Research Center for Animal HusbandryФедеральный исследовательский центр животноводства – ВИЖ им. акад. Л.К. Эрнста27042025614294003062025Copyright ©; 2025, Russian Academy of SciencesCopyright ©; 2025, Российская академия наук2025Russian Academy of SciencesРоссийская академия наукhttps://transsyst.ru/0016-6758/article/view/682200

Whole-genome RNA sequencing (RNA-Seq) was performed to compare sperm transcript profiles from bulls with different sperm productivity characteristics and cryotolerance. Ejaculates from Holstein bulls classified as having high and low cryopreservability and sperm productivity (НСS and LCS), respectively; n = 3 bulls per group) were analyzed for post-thaw motility, mitochondrial membrane potential, plasma membrane integrity, and acrosomes. Total RNA was isolated from decryopreserved spermatozoa from GSF and PSF bulls and subjected to RNA-seq (Illumina NextSeq 500 platform), and transcript arrays were assessed using DESeq2, DESeq packages in each bull's spermatozoa. Several differentially expressed genes (DEGs) related to inflammation (IRG1, MMP12), spermatogenesis (Col4A2,), and unknown function (LOC 781178, LOC618942) were identified that were preferentially downregulated in ejaculates with poor cryotolerance.

Полногеномное РНК-секвенирование было проведено для сравнения профилей транскриптов сперматозоидов быков с различными продуктивными характеристиками и способностью спермы к криоконсервации. Исследовали эякуляты быков голштинской породы, которые были классифицированы как имеющие высокую и низкую криорезистентность и высокое и низкое качество спермы (n = 3 быка в группе), оценив характеристики подвижности после размораживания, потенциал митохондриальной мембраны, целостность плазматической мембраны и акросомы. Общая РНК была выделена из декриоконсервированных сперматозоидов быков групп GSF и PSF и подвергнута РНК-секвенированию (платформа Illumina NextSeq 500), массив транскриптов оценивался с помощью пакетов DESeq2, DESeq в сперматозоидах каждого быка. Идентифицировано несколько дифференциально экспрессируемых генов (DEG), связанных с воспалением (IRG1, MMP12), сперматогенезом (Col4A2) и с неизвестной функцией (LOC 781178, LOC618942), которые преимущественно были подавлены в эякулятах с плохой криорезистентностью.

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