Russian Journal of GeneticsRussian Journal of GeneticsГенетика0016-67583034-5103The Russian Academy of Sciences66723910.31857/S0016675824070113BGNLFZКРАТКИЕ СООБЩЕНИЯКРАТКИЕ СООБЩЕНИЯShort CommunicationExpression profiles of genes involved in lignan synthesis in developing flax seedsПрофили экспрессии генов, вовлеченных в синтез лигнанов, в развивающихся семенах льнаPushkovaЕ. N.ПушковаЕ. Н.
Russian Federation
mnv-4529264@yandex.ruDvorianinovaE. М.ДворяниноваЕ. М.
Russian Federation
mnv-4529264@yandex.ruPovkhovaL. P.ПовховаЛ. П.mnv-4529264@yandex.ruRozhminaT. А.РожминаТ. А.
Russian Federation
mnv-4529264@yandex.ruNovakovskiyR. O.НоваковскийР. О.
Russian Federation
mnv-4529264@yandex.ruSigovaЕ. A.СиговаЕ. А.
Russian Federation
mnv-4529264@yandex.ruDmitrievА. А.ДмитриевА. А.
Russian Federation
mnv-4529264@yandex.ruMelnikovaN. V.МельниковаН. В.
Russian Federation
mnv-4529264@yandex.ruEngelhardt Institute of Molecular Biology, Russian Academy of SciencesИнститут молекулярной биологии им. В.А. Энгельгардта Российской академии наукFederal Research Center for Bast Fiber CropsФедеральный научный центр лубяных культур1507202460711211726022025Copyright ©; 2024, Russian Academy of SciencesCopyright ©; 2024, Российская академия наук2024Russian Academy of SciencesРоссийская академия наукhttps://transsyst.ru/0016-6758/article/view/667239

Flax seeds are the richest plant source of lignans, which prevent the development of many diseases. Secoisolariciresinol diglucoside (SDG) is the predominant lignan in seeds of the cultivated species Linum usitatissimum. We sequenced transcriptomes of flax seeds at five developmental stages for 8 varieties differing in lignan content grown under three different conditions and evaluated the expression of PLR1 and UGT74S1 genes, which play a key role in SDG synthesis. The co-expression of PLR1 and UGT74S1 genes was detected, and the expression level of these genes was observed to change tens and hundreds of times during seed development, confirming their role in SDG synthesis in flax seeds. Low temperature (16 °С) and abundant watering resulted in a shift of the maximum expression level of both genes to later dates (14th day after flowering) compared to poor watering and high temperature (24 °С) and optimal conditions (20 °С) (7th day after flowering). Meanwhile, the expression level of PLR1 and UGT74S1 genes was lower under high temperature and poor watering than under optimal conditions. No association was found between lignan content in seeds of the studied flax varieties and the expression level of PLR1 and UGT74S1 genes. Our results provide important information on the contribution of genotype and environment to the expression of key genes of SDG synthesis, which is also necessary for the development of optimal approaches to obtain lignan-rich flax seeds.

Семена льна являются богатейшим растительным источником лигнанов, препятствующих развитию многих заболеваний. Среди лигнанов в семени культивируемого вида Linum usitatissimum преобладает диглюкозид секоизоларицирезинола (SDG). Нами выполнено секвенирование транскриптомов семян льна на пяти стадиях развития для восьми сортов/линий, различающихся по содержанию лигнанов, для трех вариантов условий выращивания и проведена оценка экспрессии генов PLR1 и UGT74S1, играющих ключевую роль в синтезе SDG. Выявлены коэкспрессия генов PLR1 и UGT74S1 и изменение уровня экспрессии этих генов в десятки и сотни раз в процессе развития семян, что подтверждает их роль в синтезе SDG льняного семени. Пониженная температура (16 °С) и избыточный полив приводили к сдвигу максимального уровня экспрессии обоих генов на более поздние сроки (14-й день после раскрытия цветка) по сравнению с условиями недостаточного полива и повышенной температуры (24 °С) и оптимальными условиями (20 °С) (7-й день после раскрытия цветка). При этом при повышенной температуре и недостаточном поливе уровень экспрессии генов PLR1 и UGT74S1 был ниже, чем при оптимальных условиях. Не выявлено ассоциации между содержанием лигнанов в семенах исследованных сортов/линий льна и уровнем экспрессии генов PLR1 и UGT74S1. Наши результаты дают важную информацию о вкладе генотипа и среды в экспрессию ключевых генов синтеза SDG, что в том числе необходимо для разработки оптимальных подходов для получения семян льна с высоким содержанием лигнанов.

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