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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="brief-report" dtd-version="1.2" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">Russian Journal of Genetics</journal-id><journal-title-group><journal-title xml:lang="en">Russian Journal of Genetics</journal-title><trans-title-group xml:lang="ru"><trans-title>Генетика</trans-title></trans-title-group></journal-title-group><issn publication-format="print">0016-6758</issn><issn publication-format="electronic">3034-5103</issn><publisher><publisher-name xml:lang="en">The Russian Academy of Sciences</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">667224</article-id><article-id pub-id-type="doi">10.31857/S0016675824080114</article-id><article-id pub-id-type="edn">bfgxhu</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>КРАТКИЕ &#13;
СООБЩЕНИЯ</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>КРАТКИЕ СООБЩЕНИЯ</subject></subj-group><subj-group subj-group-type="article-type"><subject>Short Communication</subject></subj-group></article-categories><title-group><article-title xml:lang="en">Sequencing and Annotation of the Chloroplast Genome of <italic>Triticum militinae</italic> – a “Natural Mutant” of Tetraploid Wheat <italic>Triticum timopheevii</italic> Zhuk.</article-title><trans-title-group xml:lang="ru"><trans-title>Секвенирование и аннотация хлоропластного генома <italic>Triticum militinae</italic> – “естественного мутанта” тетраплоидной пшеницы <italic>Triticum timopheevii</italic> Zhuk</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Kuluev</surname><given-names>A. R.</given-names></name><name xml:lang="ru"><surname>Кулуев</surname><given-names>А. Р.</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>kuluev.azat91@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Matniyazov</surname><given-names>R. T.</given-names></name><name xml:lang="ru"><surname>Матниязов</surname><given-names>Р. Т.</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>kuluev.azat91@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Kuluev</surname><given-names>B. R.</given-names></name><name xml:lang="ru"><surname>Кулуев</surname><given-names>Б. Р.</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>kuluev.azat91@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Privalov</surname><given-names>L. Yu.</given-names></name><name xml:lang="ru"><surname>Привалов</surname><given-names>Л. Ю.</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>kuluev.azat91@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><name-alternatives><name xml:lang="en"><surname>Chemeris</surname><given-names>A. V.</given-names></name><name xml:lang="ru"><surname>Чемерис</surname><given-names>А. В.</given-names></name></name-alternatives><address><country country="RU">Russian Federation</country></address><email>kuluev.azat91@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="en">Institute of Biochemistry and Genetics – Subdivision of the Federal State Budgetary Scientific Institution of the Ufa Federal Research Center of the Russian Academy of Sciences</institution></aff><aff><institution xml:lang="ru">Институт биохимии и генетики – обособленное структурное подразделение Федерального государственного бюджетного научного учреждения Уфимского федерального исследовательского центра Российской академии наук</institution></aff></aff-alternatives><pub-date date-type="pub" iso-8601-date="2024-08-15" publication-format="electronic"><day>15</day><month>08</month><year>2024</year></pub-date><volume>60</volume><issue>8</issue><fpage>118</fpage><lpage>121</lpage><history><date date-type="received" iso-8601-date="2025-02-26"><day>26</day><month>02</month><year>2025</year></date></history><permissions><copyright-statement xml:lang="en">Copyright ©; 2024, Russian Academy of Sciences</copyright-statement><copyright-statement xml:lang="ru">Copyright ©; 2024, Российская академия наук</copyright-statement><copyright-year>2024</copyright-year><copyright-holder xml:lang="en">Russian Academy of Sciences</copyright-holder><copyright-holder xml:lang="ru">Российская академия наук</copyright-holder></permissions><self-uri xlink:href="https://transsyst.ru/0016-6758/article/view/667224">https://transsyst.ru/0016-6758/article/view/667224</self-uri><abstract xml:lang="en"><p><italic>Triticum</italic> <italic>militinae</italic> Zhuk. et Migusch. – tetraploid wheat with the GAA genome, considered a natural naked mutant of <italic>T</italic><italic>. </italic><italic>timopheevii</italic> Zhuk. Previously, experiments were conducted for this wheat to study the karyotype and crossing characteristics. To clarify its origin and relationship with other representatives of the wheat family, analysis of the chloroplast genome of <italic>T</italic><italic>. </italic><italic>militinae</italic>, which has previously remained unstudied, is of great interest.<bold> </bold>The sucrose gradient method was used to isolate chloroplast DNA from leaves. Sequencing was performed using the FASTASeq 300 Sequencing Kit V1.0 100 M reads/flow cell on a Genolab M sequencer (GeneMind, China). For the first time, we sequenced and annotated the complete chloroplast genome of <italic>T</italic><italic>. </italic><italic>militinae</italic> with a size of 135898 bp. In the structure of the plastid genome there are a pair of inverted repeats with a size of 21552 bp each, the region of a small single copy (SSC) – 12791 bp and the region of a large single copy (LSC) – 80003 bp. As part of the chloroplast genome of <italic>T</italic><italic>. </italic><italic>militinae</italic> 132 structural genes were found, of which 85 protein-coding genes, 31 tRNAs and 4 rRNA genes.</p></abstract><trans-abstract xml:lang="ru"><p><italic>Triticum</italic><italic> </italic><italic>militinae</italic><italic> </italic>Zhuk. et Migusch. – тетраплоидная пшеница с геномом GAA, считается естественным голозерным мутантом <italic>Triticum</italic><italic> </italic><italic>timopheevii</italic> Zhuk. Ранее для этой пшеницы проводились эксперименты по изучению кариотипа и особенностей скрещивания. Для выяснения происхождения и родства <italic>T. </italic><italic>militinae</italic> с другими представителями пшеницевых большой интерес представляет анализ ее хлоропластного генома<italic>, </italic>который оставался неизученным. Нами впервые было проведено секвенирование и аннотация полного хлоропластного генома<italic> </italic><italic>T. </italic><italic>militinae, </italic>размер которого оказался равным 135898 пн. В структуре пластома этой пшеницы имеются пара инвертированных повторов размером 21552 пн каждый, область маленькой единичной копии (SSC) – 12791 пн, и область большой единичной копии (LSC) – 80003 пн. В составе хлоропластного генома<italic> </italic><italic>T. </italic><italic>militinae </italic>аннотировано 132 структурных гена, из которых 85 белок-кодирующих, 31 ген тРНК и четыре гена рРНК.</p></trans-abstract><kwd-group xml:lang="en"><kwd>wheat</kwd><kwd>Triticum militinae</kwd><kwd>Triticum timopheevii</kwd><kwd>chloroplast genome</kwd><kwd>sequencing</kwd></kwd-group><kwd-group xml:lang="ru"><kwd>пшеница</kwd><kwd>Triticum militinae</kwd><kwd>Triticum timopheevii</kwd><kwd>хлоропластный геном</kwd><kwd>секвенирование</kwd></kwd-group><funding-group><award-group><funding-source><institution-wrap><institution xml:lang="ru">Российский научный фонд (грант)</institution></institution-wrap><institution-wrap><institution xml:lang="en">Russian Science Foundation (grant)</institution></institution-wrap></funding-source><award-id>23-24-00275</award-id></award-group></funding-group></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><mixed-citation>Жуковский П.М., Мигушова Э.Ф. 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