Russian Journal of GeneticsRussian Journal of GeneticsГенетика0016-67583034-5103The Russian Academy of Sciences66701810.31857/S0016675823120056QDLMALОБЩАЯ ГЕНЕТИКАRegulation of Uni-Strand and Dual-Strand piRNA Clusters in Germ and Somatic Tissues in Drosophila melanogaster under Control of rhinoРегуляция одноцепочечных и двуцепочечных кластеров piРНК в герминальных и соматических тканях Drosophila melanogaster зависит от гена rhinoMilyaevaP. A.МиляеваП. А.nefedova@mail.bio.msu.ruLavrenovA. R.ЛавреновА. Р.nefedova@mail.bio.msu.ruKuzminI. V.КузьминИ. В.nefedova@mail.bio.msu.ruKimA. I.КимА. И.nefedova@mail.bio.msu.ruNefedovaL. N.НефедоваЛ. Н.nefedova@mail.bio.msu.ruLomonosov Moscow State UniversityМосковский государственный университет им. М.В. ЛомоносоваFaculty of Biology, Shenzhen MSU-BIT UniversityБиологический факультет, университет МГУ-ППИ в ШэньчжэнеSevertsov Institute of Ecology and Evolution of Russian Academy of SciencesИнститут проблем экологии и эволюции им. А.Н. Северцова Российской академии наук0112202359121372138126022025Copyright ©; 2023, П.А. Миляева, А.Р. Лавренов, И.В. Кузьмин, А.И. Ким, Л.Н. НефедоваCopyright ©; 2023, П.А. Миляева, А.Р. Лавренов, И.В. Кузьмин, А.И. Ким, Л.Н. Нефедова2023П.А. Миляева, А.Р. Лавренов, И.В. Кузьмин, А.И. Ким, Л.Н. НефедоваП.А. Миляева, А.Р. Лавренов, И.В. Кузьмин, А.И. Ким, Л.Н. Нефедоваhttps://transsyst.ru/0016-6758/article/view/667018

Drosophila melanogaster is a common genetic object for research of RNA-interference pathways and mobile elements regulation. Nowadays taking a part in control of retrotransposon expression the system of piRNA-interfecence well studied in ovary tissues. It is strongly believed that D. melanogaster piRNA-interference is used for retrotransposon suppression only in gonads, and two distinct pathways of piRNA biogenesis exist. Both mechanisms use transcripts of piRNA-clusters (accumulations of truncated and defect mobile elements copies): from unstrand clusters in the first case and from dualstrand clusters in the second, transcribed with one or both DNA chains correspondingly. It is well-known that proper dualstrand clusters function depends on the gene rhino, while unistrand clusters are transcribed rhino-independent and transcripts are spliced. In this paper we show that rhino participates in unistrand flamenco transcripts splicing and the piRNA-interference significance for regulation of several retrotransposons not only in gonads, but in other organs.

Drosophila melanogaster – широко используемый объект генетики для изучения механизмов РНК-интерференции и регуляции мобильных генетических элементов (МГЭ), в частности системы piРНК-интерференции. Считается, что у D. melanogaster система piРНК-интерференции работает только в гонадах и существуют два пути биогенеза piРНК – с помощью одноцепочечных и двуцепочечных кластеров, содержащих дефектные копии МГЭ, транскрибируемых с одной или двух цепей соответственно. Предполагается, что функционирование двуцепочечных кластеров зависит от функции гена rhino, в то время как одноцепочечные кластеры транскрибируются и затем сплайсируются независимо от rhino. В настоящей работе мы показали, что ген rhino участвует в регуляции сплайсинга одноцепочечного кластера flamenco. Более того, система piРНК-интерференции необходима для регуляции некоторых ретротранспозонов не только в гонадах, но и в других органах.

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