Russian Journal of GeneticsRussian Journal of GeneticsГенетика0016-67583034-5103The Russian Academy of Sciences66690410.31857/S0016675823010125CNOZBDОБЩАЯ ГЕНЕТИКАThe Effect of the aPKC Gene Encoding Atypical Protein Kinase C on the Lifespan of Drosophila melanogaster Depends on the Expression Level of Protein Kinase GSK3Влияние гена aPKC, кодирующего атипичную протеинкиназу C, на продолжительность жизни D. melanogaster зависит от уровня экспрессии протеинкиназы GSK3TrostnikovM. V.ТростниковМ. В.mikhail.trostnikov@gmail.comVeselkinaE. R.ВеселкинаЕ. Р.mikhail.trostnikov@gmail.comAndreevY. A.АндреевЮ. А.mikhail.trostnikov@gmail.comKhryachkovaA. Y.ХрячковаА. Ю.mikhail.trostnikov@gmail.comRoshinaN. V.РощинаН. В.mikhail.trostnikov@gmail.comPasyukovaE. G.ПасюковаЕ. Г.mikhail.trostnikov@gmail.comInstitute of Molecular Genetics of the National Research Centre “Kurchatov Institute”Институт молекулярной генетики Национального исследовательского центра “Курчатовский институт”Vavilov Institute of General Genetics, Russian Academy of SciencesИнститут общей генетики им. Н.И. Вавилова Российской академии наук01012023591263826022025Copyright ©; 2023, М.В. Тростников, Е.Р. Веселкина, Ю.А. Андреев, А.Ю. Хрячкова, Н.В. Рощина, Е.Г. ПасюковаCopyright ©; 2023, М.В. Тростников, Е.Р. Веселкина, Ю.А. Андреев, А.Ю. Хрячкова, Н.В. Рощина, Е.Г. Пасюкова2023М.В. Тростников, Е.Р. Веселкина, Ю.А. Андреев, А.Ю. Хрячкова, Н.В. Рощина, Е.Г. ПасюковаМ.В. Тростников, Е.Р. Веселкина, Ю.А. Андреев, А.Ю. Хрячкова, Н.В. Рощина, Е.Г. Пасюковаhttps://transsyst.ru/0016-6758/article/view/666904

Drosophila melanogaster shaggy and aPKC genes encode highly conserved GSK3 (Glycogen Syntase Kinase 3) and aPKC (Protein Kinase C) proteinkinases that play key roles in many cellular processes. We previously demonstrated that changes in shaggy expression in neurons affect lifespan. In this article we show that changing the expression of the aPKC gene in neurons also affects lifespan. Changing the expression of the two protein kinases in all male or female neurons and in male motoneurons led to changes in lifespan, indicating that aPKC has no effect on GSK3 and GSK3 has a possible inhibitory effect on aPKC. At the same time, changes in the expression of two protein kinases in female motoneurons led to changes in lifespan, indicating the existence of a still unclear mechanism of interaction between these proteins. The elucidation of the mechanisms of interaction between aPKC, GSK3 and their other partners will deepen and expand our understanding of the causes of longevity and the ways to extend life.

Гены shaggy и aPKC Drosophila melanogaster кодируют высококонсервативные протеинкиназы GSK3 (Glycogen Syntase Kinase 3) и aPKC (Protein Kinase C), играющие ключевую роль во многих клеточных процессах. Ранее мы продемонстрировали, что изменение экспрессии гена shaggy в нейронах влияет на продолжительность жизни. В настоящей статье показано, что изменение экспрессии гена aPKC в нейронах также влияет на продолжительность жизни. Изменение экспрессии двух протеинкиназ во всех нейронах самцов и самок и в мотонейронах самцов приводило к изменению продолжительности жизни, свидетельствующему об отсутствии влияния aPKC на GSK3 и возможном ингибирующем действии GSK3 на aPKC. В то же время изменение экспрессии двух протеинкиназ в мотонейронах самок приводило к изменению продолжительности жизни, свидетельствующему о существовании пока неясного механизма взаимодействия между этими белками. Выяснение механизмов взаимодействия между aPKC, GSK3 и другими их партнерами позволит углубить и расширить наши представления о причинах долголетия и способах продления жизни.

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